Sarcomere length measurement permits high resolution normalization of muscle fiber length in architectural studies
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چکیده
منابع مشابه
Sarcomere length measurement permits high resolution normalization of muscle fiber length in architectural studies.
The use of sarcomere length to normalize fiber length in architectural studies is commonly practiced but has not been explicitly validated. Using mouse hindlimb muscles as a model system, ankle joints were intentionally set to angles ranging from 30 degrees to 150 degrees and their muscles fixed. Tibialis anterior (TA), extensor digitorum longus (EDL) and soleus muscles were removed and their r...
متن کاملIntraoperative single-site sarcomere length measurement accurately reflects whole-muscle sarcomere length in the rabbit.
PURPOSE To compare single-site intraoperative sarcomere length values with sarcomere lengths measured from systematic sampling of the entire transferred muscle. METHODS The tendon of the rabbit second toe extensor muscle was transposed to the ankle extensor retinaculum under levels of stretch over the sarcomere length range of about 2.5 microm to about 4.0 microm. Intraoperative sarcomere len...
متن کاملDependence of shortening heat on sarcomere length in frog muscle and fiber bundles.
The relation between shortening heat and sarcomere length was studied using fiber bundles from frog semitendinosus muscles as well as using whole muscles. The initial sarcomere length was varied between 2.0 and 3.66 micron. Shortening heat was estimated as the excess heat produced after a rapid isovelocity release in a 3 sec tetanus at 0 degrees C. The isometric control heat was measured in the...
متن کاملCooperative activation in cardiac muscle: impact of sarcomere length.
This study was undertaken to determine the impact of sarcomere length (SL) on the level of cooperative activation of the cardiac myofilament at physiological [Mg2+]. Active force development was measured in skinned rat cardiac trabeculae as a function of free [Ca2+] at five SLs (1.85-2.25 microm; 1 mM free [Mg2+]; 15 degrees C). Only muscle preparations with minimal force rundown during the ent...
متن کاملQuantification of sarcomere length distribution in whole muscle frozen sections.
Laser diffraction (LD) is a valuable tool for measuring sarcomere length (Ls), a major determinant of muscle function. However, this method relies on few measurements per sample that are often extrapolated to whole muscle properties. Currently it is not possible to measure Ls throughout an entire muscle and determine how Ls varies at this scale. To address this issue, we developed an actuated L...
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ژورنال
عنوان ژورنال: Journal of Experimental Biology
سال: 2005
ISSN: 1477-9145,0022-0949
DOI: 10.1242/jeb.01763